The FFS Data Processor (FFSDP) software is developed to analyze the data obtained from Fluorescence Fluctuation Spectroscopy measurements.
FCS, FCCS, TIR-FCS, PCH, PCMH, FCA, TIFCA and Coincidence analysis are supported. The FFSDP can import both raw data traces and directly exported from the equipment characteristics such as correlation functions and photon counting distributions. ConfoCor, ConfoCor2, ConfoCor3 (Carl Zeiss GmbH), ISS ALBA, PicoHarp 300, Time Harp 200 (PicoQuant GmbH) (new) and B&H SPC data formats are supported. Software calculates autocorrelation (AC) and crosscorrelation (CC) functions, photon counting distribution (PCD), inter-event time distribution (IED), fluorescence factorial cumulants (FFC) and coincidence histograms from imported binary raw data. Global fit of AC, CC, PCD, FFC with linking parameters is supported. In addition a number of characteristics can be analyzed sequentially one by one. Analysis of coincidence histograms with calculation of separation value and coincidence burst counting can be also performed. Software package contains a set of most popular models for correlation functions: pure-diffusion model, triplet-state model, conformational model, protonation model and flow model. 2D and 3D free, anomalous and confined diffusion types are supported. Corrections for out-of-focus emission, dead time, afterpulses, diffusion and triplet processes, set of most popular brightness profile approximations including Gauss-Lorenzian and FIDA-like polinomial are supported in PCH and FCA analysis. Software provides quick and easy creation and saving of user-defined models. Besides standard graphs displaying the analysis results, software can show and fit to a model a graphical dependence of fit parameters on any variable characterizing the source data.